The wine industry faces significant threats from authenticity, adulteration and fraud, particularly through the illicit addition of exogenous sugars to grape must to artificially boost alcohol content. However, stable isotope ratio analysis (SIRA) techniques, including nuclear magnetic resonance (SNIF-NMR) and isotope ratio mass spectrometry (IRMS), offer a powerful means of detecting such adulterations. By combining these techniques, researchers can determine the botanical and geographical origin of wine samples, providing a robust tool for authenticity testing in line with Regulation (EU) 1308/2013.