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New set of GM cotton certified reference materials released

15 July 2021

The JRC developed a new set of GMO certified reference materials (CRMs). The five CRMs are certified for their mass fraction of GHB811 cotton. After authorisation of the GMO event in food and feed products for the EU market, the CRMs will enable the use of the GMO quantification method for GHB811 cotton and the implementation of EU legislation on GMO labelling. 

The GHB811 cotton (with unique identifier code BCS-GH811-4), developed by BASF Agricultural Solutions Seed LLC (US), has been modified for resistance to the herbicides glyphosate and isoxaflutole. The double mutant 5-enol pyruvylshikimate-3-phosphate synthase (2mepsps) gene that encodes for the 2mEPSPS protein allows resistance to glyphosate. Tolerance to isoxaflutole herbicides is achieved by the expression of a modified p hydroxyphenyl pyruvate-dioxygenase (HPPD) protein, encoded by the hppdPf W336 gene, derived from the soil bacterium Pseudomonas fluorescens [1].
EU Regulation No 1829/2003 [2] requests that reference materials are made available for GMO events authorised for the EU food and feed market. BASF asked the JRC to produce a set of CRMs for the GHB811 cotton event. It is released under the codes ERM-BF442a, ERM-BF442b ERM-BF442c ERM-BF442d ERM-BF442e  with nominal GHB811 cotton concentration levels of 0 %, 0.1 %, 1 %, 10 % and 100 %.
ERM-BF442a to ERM-BF442e will enable GMO testing and official control laboratories to quantify GHB811 cotton in food and feed products. In combination with the measurement method validated by the EURL-GMFF [3], this set of CRMs is establishing the arbitrary reference system required for quantification of GHB811 cotton.


[2] Regulation (EC) No 1829/2003 of the European Parliament and of the Council of 22   September 2003 on genetically modified food and feed. Off. J. Eur. Union, L 268:1-28
[3] Homepage of the European Union Reference Laboratory for GM food and feed; http://gmo-crl.jrc.ec.europa.eu/


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