New set of GM soya bean certified reference materials released
30 March 2021A new set of GMO certified reference materials (CRMs) was developed by the JRC. This set is composed of five CRMs, certified for their mass fraction of GMB151 soya bean. After authorisation of the GMO event in food and feed products for the EU market, the CRMs will enable the application of GMO quantification methods implementing EU legislation on GMO labelling.
BASF Plant Science GmbH (Ludwigshafen, Germany) developed the genetically modified (GM) GMB151 soya bean event (unique identifier code BCS-GM151-6) as a transgenic crop conferring cyst nematode resistance and herbicide tolerance to 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors. The GMB151 soya bean contains the Bacillus thuringiensis Cry14Ab1 gene for resistance to nematode plant parasites, such as soya bean cyst nematode. For herbicide tolerance, it has a Pseudomonas fluorescens HPPD gene with point mutations in the C-terminal to allow for and enhance tolerance to HPPD-inhibitor herbicides, such as isoxaflutole and mesotrione [1].EU Regulation No 1829/2003 [2] requests that reference materials are made available for GMO events authorised for the EU food and feed market. BASF asked the JRC to produce a set of CRMs for the GMB151 soya bean event. It is released under the code ERM-BF443a to ERM-BF443e, with nominal GMB151 soya bean concentration levels of 0 %, 0.1 %, 1 %, 10 % and 100 %.
The set of CRMs ERM-BF443a to ERM-BF443e will enable GMO testing and official control laboratories to quantify GMB151 soya bean in food and feed product. In combination with the measurement method validated by the EURL-GMFF [3], this set of CRMs is establishing the arbitrary reference system required for quantification of GMB151 soya bean.
[1]Biosafety Clearing-House, http://bch.cbd.int/database/record.shtml?documentid=115763
[2]Regulation (EC) No 1829/2003 of the European Parliament and of the Council of 22 September 2003 on genetically modified food and feed. Off. J. Eur. Union, L 268:1-28
[3]Homepage of the European Union Reference Laboratory for GM food and feed; http://gmo-crl.jrc.ec.europa.eu/
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